Spine Fit

High performance liquid chromatography is a technique that has undergone a multitude of changes from the time it was first devised by tswett. It is a variant of the column chromatography technique and is widely used in the biochemistry and analytic s laboratory. It consists of a column with a diameter of a few mm which is packed with an immobilized material and a mobile solution containing the sample to be analyzed is passed through it. There is a detector such as a Mass spectrometer or a fluorescence analyzer which helps in the quantification of the materials present. This devise can be used to effectively identify materials based on their polarity, size, mass or their affinity towards another molecule.

This technique ranks among one of the worlds best methods for the identification of molecules and therefore plays an important role in many fields such as clinical analysis, drug detection, environmental science, Food and bio process industries. Like most modern tools this too has been incorporated into an automated system wherein a little bit of computer programming is enough to detect a variety of compounds with ease. Therefore it can be understood that there is a huge amount of research that has been performed in order to devise such accurate devises with the potential to influence a variety of fields.